NACK的表达水平对非小细胞肺癌预后的影响

摘要

目的:探讨NACK在非小细胞肺癌(non-small cell lung cancer,NSCLC)中的表达及其与临床病理特征的关系.方法:采用RT-PCR及Western blot法对35例非小细胞肺癌患者的肺癌组织及癌旁组织的NACK表达水平进行定量分析,以及NACK的表达与非小细胞肺癌临床病理特征的关系,通过生存曲线分析随访病人的总体生存率(OS)和无病生存率(DFS).结果:非小细胞肺癌患者NACK相对表达水平高于正常对照组(P<0.05),其表达与临床分期、分化程度及淋巴结转移显著相关(P<0.05),而与患者的年龄、性别、肿瘤大小、抽烟、病理类型无关(P>0.05).随访3年NACK高表达的病人总体生存率(OS)和无病生存率(DFS)分别为30.7%和34.6%,而低表达者分别为OS 77.8%和DFS 81.8%,两组间差异有显著统计学意义(P<0.001).结论:我们认为NACK的高表达可以作为预测NSCLC预后不良的高危因素,为以后深入研究NACK-Notch调控机制和探索新的治疗靶点提供重要基础.%Objective:To evaluate the value of expression of NACK in non-small cell lung cancer (NSCLC) and to explore its correlation with clinicopathological parameters in NSCLC.Methods:NACK expression in tumor tissue and normal lung tissue of 35 cases of NSCLC was detected by RT-PCR and Western blot and its correlation with clinicopathological parameters were analyzed.We observed that the cumulative 4-year overall survival rate (OS) and disease free survival (DFS) of patients with different level of NACK expression.Results:NACK was discovered highly expressed both at mRNA and protein levels in the NSCLC tissue samples compared with the paired adjacent normal tissues (P<0.05).The expression of NACK was significantly correlated with the degree of differentiation (P=0.034),lymphatic metastasis (N stage) (P=0.007) and clinical stage (P=0.014).The level of NACK expression was not associated with gender,age,smoking,tumor size or histological type (P>0.05).The cumulative 3-year overall survival rate (OS) and disease free rate (DFS) of patients with high level of NACK detection were 30.7% and 34.6%,respectively,when compared to low NACK expression patients (77.8% and 81.8%,respectively).Conclusion:We regard the high expression of NACK as a risk factor predicting poor NSCLC prognosis.NACK may be an attractive target to help develop novel therapeutic methods against NSCLC.Insightful studies are still needed to disclose other signaling pathways involved in regulating NSCLC.