Serglycin稳定干扰对鼻咽癌高转移细胞顺铂敏感性的影响及机制研究

摘要

Objective:To observe the effect of Serglycin on the stability of high metastatic cells of nasopharyngeal carcinoma with high sensitivity to cisplatin,and to explore the mechanism of interference.Methods:Serglycin stable interference of nasopharyngeal carcinoma with high metastatic cell lines were divided into three groups:experimental group 5 -8f KDA and 5 -8f KDC and control group 5 -8f scrambled.To detect Serglycin protein expression,to ob-serve the parental and stable plant cell morphology.MTT assay was used to detect the absorbance,draw the curve of the proliferation under the effect of cisplatin.To detect cell viability,stem cell gene expression,and content of Vimen-tin and E-cadherin.Results:In the experimental group,the expression of Serglycin gene was inhibited.The Serglycin gene expression in the control group was more.The difference was significant,with statistical significance(P<0.05). The secreted Serglycin protein in the experimental group was significantly decreased,and the expression of Serglycin in the control group was higher.The cell morphology of the stable group was round or polygon,and the control group cells were spindle shaped.MTT was found to be significantly faster in the control group than in the third day after cis-platin,and there was statistical significance(P<0.05).Compared with the 5 -8f scrambled group,the cell survival rate of the experimental group was significantly decreased,and the cell survival rate was statistically significant(P<0.05).In stable group,the expression of gene expression was significantly decreased,and the difference was signifi-cant(P<0.05).Serglycin can promote the expression of Vimentin protein and inhibit the expression of E-cadherin protein in tumor tissue.Conclusion:Serglycin can increase the sensitivity of nasopharyngeal carcinoma cells with high metastatic cells to cisplatin.%目的：观察鼻咽癌高转移细胞5－8f的顺铂敏感性受Serglycin稳定干扰的影响，并对干扰机制进行初步探究。方法：建立Serglycin 稳定干扰的鼻咽癌高转移细胞株，分为三组：实验组5－8f KDA和5－8f KDC及对照组5－8f scrambled。测定Serglycin蛋白的表达情况；观察亲代及稳定株细胞形态；MTT法检测吸光度，绘制顺铂作用下的增殖曲线；测定细胞存活率；测定干细胞基因表达量；测定Vimentin 与 E－cadherin 含量。结果：实验组Serglycin基因表达被抑制，对照组Serglycin基因表达较多，差异具有统计学意义（P＜0．05）。实验组的分泌性Serglycin蛋白明显减少，对照组的Serglycin分泌蛋白表达较多。稳定组细胞形态呈圆形或多边形，对照组细胞为长梭形。MTT检测发现癌细胞受顺铂作用后第三天开始，对照组细胞吸光度增加较稳定组细胞明显快，差异有统计学意义（P＜0．05）。与5－8f scrambled组相比，实验组的细胞存活率明显下降，差异具有统计学意义（P＜0．05）。稳定组细胞干细胞基因表达明显降低，差异具有统计学意义（P＜0．05）。肿瘤组织中 Serglycin 能促进Vimentin 蛋白的表达，抑制E－cadherin 蛋白的表达。结论：Serglycin 稳定干扰可以增加鼻咽癌高转移细胞对顺铂的敏感性。