Livin 蛋白在支气管肺癌中的表达及与 Bcl －2的关系

摘要

目的：探讨 Livin 蛋白在原发性支气管肺癌组织的表达及其与 Bcl －2蛋白之间的关系。方法：采用免疫组化 SP 法检测 Livin 与 Bcl －2在支气管肺癌组织和癌旁5cm 以上正常肺组织中蛋白的表达情况。结果：肺癌组的 Livin 蛋白和 Bcl －2蛋白阳性表达率均高于对照组（均 P ＜0．05）。男性肺癌患者 Livin 蛋白的表达阳性率为62．96％，女性为40．00％，两者相比差异有统计学意义（P ＜0．05）。有淋巴结转移的肺组织中Livin 蛋白和 Bcl －2蛋白的阳性表达率较高（78．95％ vs 36．36％，68．42％ vs 27．27％，均 P ＜0．05）。肺鳞癌组织 Bcl －2基因蛋白的阳性表达率为58．82％，显著高于肺腺癌组织中 Bcl －2基因蛋白表达（20．45％），差异有统计学意义（P ＜0．05）。吸烟指数≥400的肺癌患者 Livin 蛋白及 Bcl －2蛋白表达阳性率均高于吸烟指数＜400的患者，差异有统计学意义（P ＜0．05）。支气管肺癌的组织分化程度、临床分期对 Livin 蛋白和 Bcl －2蛋白的阳性表达率的影响均无统计学差异。Livin 蛋白与 Bcl －2蛋白的表达呈正相关。结论：Livin 蛋白与Bcl －2蛋白在支气管肺癌的异常表达，有望成为肿瘤诊断及基因治疗的新靶点。Livin 蛋白与凋亡相关蛋白Bcl －2的异常表达在支气管肺癌癌变中有协调作用。%Objective:To study the expression of Livin and it's relationship with expression of Bcl -2 in primary lung cancer.Methods:The protein expression of Livin and Bcl -2 gene in paraffin specimens of lung cancer and specimens of normal lung tissues were detected by SP immunohistochemistry.Results:Livin and Bcl -2 protein ex-pression showed high level in lung cancer specimens and were negative staining in the normal lung tissues.The posi-tive rate of Livin protein expression in male lung cancer specimens(62.96%)was higher than that in female lung tis-sues(40.00%,P <0.05).The positive rates of Livin and Bcl -2 protein expression in specimens with lymph node me-tastasis (78.95% and 68.42%)were higher than in tissues without lymph node metastasis(36.36% and 27.27%,P<0.05).The protein expression of Bcl -2 gene in squamous cell carcinoma lung tissues(58.82%)was higher than that in adenocarcinoma lung tissues(20.45%).The positive rates of expressions of Livin and Bcl -2 protein were higher in patients smoking index ≥400 than patients with <400,the differences were significant(P <0.05).Livin protein expression in lung cancer had no obvious relationship with the grade and clinical stage.Livin protein expres-sion was positively related to Bcl -2 protein expression in primary lung cancer.Conclusion:Aberrant expression may be a new target for diagnosis and gene treatment of primary lung cancer.The aberrant expression of Livin and apopto-sis related gene Bcl -2 may play synergetic roles in process of carcinogenesis of lung cancer.