β－arrestin2基因转染对人肺腺癌细胞 A549的凋亡影响

摘要

Objective:To determine the effects of exogenous β-arrestin2 on human lung adenocarcinoma cells A549.Methods:pcDNA3.1 -β-arrestin2 and pcDNA3.1 were introduced into A549 cells by lipofectin transfec-tion,and the A549 cells stably expressing β-arrestin2 gene were established by G418 selection.The expression of β-arrestin2 was detected by qRT -PCR and Western blot.Apoptosis of cells was detected by flow cytometry.Results:A549 cells stably expressing β-arrestin2 protein were established by lipofection mediated transfection and selected for further study.Compared with the nontransfected and vector transfected cells,the positive clone cells grew more slowly(P <0.05).Conclusion:β-arrestin2 gene might promote the apoptosis of A549 cells.%目的：观察外源性β－arrestin2基因对人肺腺癌细胞株 A549凋亡的影响。方法：将真核表达重组体pcDNA3．1－β－arrestin2质粒和空载体 pcDNA3．1质粒采用脂质体转染技术分别导入人肺腺癌 A549细胞，然后采用 G418筛选方法获得稳定转染的细胞系，采用 qRT －PCR 和 Western blot 分别在基因水平和蛋白水平检测β－arrestin2基因的表达。采用流式细胞仪分析转染细胞的凋亡情况。结果：经脂质体转染和筛选，建立了稳定表达β－arrestin2基因的 A549细胞系。转染β－arrestin2基因的细胞生长速度较未转染组和转染空白载体组相比明显减慢（P ＜0．05）。结论：β－arrestin2基因可促进人肺腺癌细胞 A549的细胞凋亡。