DUSP10对人卵巢癌A2780细胞迁移及侵袭的影响

摘要

目的：探讨DUSP10在浆液性卵巢癌组织中的表达情况及其与卵巢癌细胞迁移及侵袭能力的关系。方法：实时荧光定量PCR方法检测浆液性卵巢腺癌和正常输卵管伞端组织中 DUSP10的表达情况；转染DUSP10过表达和干扰质粒至人卵巢癌A2780细胞，划痕实验和Transwell实验观察癌细胞迁移及侵袭能力的变化。结果：DUSP10在浆液性卵巢癌组织的表达低于正常输卵管伞端组织（ P﹤0.05）。与对照组相比，转染DUSP10过表达质粒后A2780细胞迁移及侵袭能力受到抑制，转染DUSP10干扰质粒后A2780细胞迁移及侵袭能力增强。结论：DUSP10在浆液性卵巢癌组织中低表达；DUSP10可抑制人卵巢癌A2780细胞的迁移及侵袭能力。%Objective:To investigate the expression of DUSP10 in serous ovarian cancer tissues and its relationship with ovarian cancer cell migration and invasion ability. Methods:Real-time quantitative PCR was performed to de-tect the expression of DUSP10 in serous ovarian cancer and normal fimbria of fallopian tubes. Wound healing assay and Transwell assay were used to observe the migration and invasion ability of A2780 cells by transfected DUSP10 overexpression plasmid and interference plasmid. Results:The mRNA expression of DUSP10 was significantly down-regulated in serous ovarian cancers than normal fimbria of fallopian tubes(P﹤0. 05). The migration and invasion a-bility of A2780 cells were significantly inhibited after overexpression of DUSP 1 0 ,however ,silencing DUSP10 can promote cell migration and invasion. Conclusion:The expression of DUSP10 was significantly down-regulated in se-rous ovarian cancer,DUSP10 can inhibit the migration and invasion ability of ovarian cancer A2780 cells.