HSP27在胃印戒细胞癌组织中的表达及其与β-catenin和Vimentin的关系

摘要

Objective:To discuss the expression of HSP27 and its correlation with β-catenin and Vimentin in Signet-ring cell carcinoma( SRCC)of the stomach. Methods:To choose 70 samples of gastroscopic biopsies,inclu-ding 30 SRCC,30 poor differentiated adenocarcinoma and 10 chronic gastritis. Expression of HSP27,β-catenin and Vimentin was detected in paraffin-embedded specimens using immunohistochemical method. Results:Expression of HSP27 and Ectopic expression of β-catenin in SRCC were 80% and 40%,there were significantly differences with poor differentiated adenocarcinoma(P﹤0. 05),and there was a negative correlation between HSP27 and Ectopic ex-pression ofβ-catenin(P﹤0. 05). The rate of normal expression of β-catenin were 46. 7% and 23. 3% in SRCC and poor differentiated adenocarcinoma,there were no significantly differences between them(P﹥0. 05). And there was no correlation between HSP 2 7 and normal expression of β - catenin . The rates of expression of Vimentin were 33. 3% and 53. 3% in SRCC and poor differentiated adenocarcinoma,there were no significantly differences be-tween them(P﹥0. 05),and there was no correlation with HSP27 andβ-catenin(P﹥0. 05). Conclusion:HSP27,β-catenin and Vimentin protein all have abnormal expression in SRCC and poor differentiated adenocarcinoma. HSP27 protein expression in SRCC is higher than poor differentiated adenocarcinoma,but the rate of abnormal expres-sion ofβ-catenin and Vimentin protein is lower than poor differentiated adenocarcinoma. HSP27,β-catenin and Vi-mentin may be involved in the difference biological behaviour between SRCC and poor differentiated adenocarcinoma.%目的：检测热休克蛋白27（HSP27）、β-连接素（β-catenin）及波形蛋白（Vimentin）在胃印戒细胞癌中的表达，并分析三者之间的关系。方法：选取70例胃镜活检组织，包括印戒细胞癌（ SRCC）30例，低分化腺癌30例，黏膜慢性炎症10例。免疫组化检测HSP27、β-catenin及Vimentin蛋白的表达。结果：印戒细胞癌HSP27表达率及β-catenin异位表达率分别为80%、40%，与低分化腺癌（53.3%、66.7%）比较差异具有统计学意义（P﹤0.05）。印戒细胞癌中HSP27与β-catenin异位表达率间存在负相关（P﹤0.05）。印戒细胞癌β-catenin正常表达率（46.7%）与低分化腺癌（23.3%）之间差异无统计学意义（ P﹥0.05），与HSP27表达无相关性（P﹥0.05）。印戒细胞癌Vimentin表达率（33.3%）与低分化腺癌（53.3%）比较无统计学差异（P﹥0.05），并与β-catenin的异位表达及HSP27之间无相关性（ P﹥0.05）。结论：HSP27、β-catenin及Vimen-tin在印戒细胞癌及低分化腺癌中均有不同程度的异常表达。印戒细胞癌HSP27的表达显著高于低分化腺癌，而β-catenin的异位表达显著低于低分化腺癌，并且Vimentin的表达率与低分化腺癌比较，处于较低水平。在印戒细胞癌及低分化腺癌中，三者的表达差异可能与肿瘤的不同生物学行为有关。