沉默信息调节因子-1及上皮性钙黏附素在胃癌中的表达及其相关性

摘要

目的:探讨沉默信息调节因子-1(silence signal regulating factor-1,SIRT-1)和上皮性钙黏附素(epithelial cadherin,E-cadherin)在胃癌组织中的表达及其相关性.方法:采用HE染色观察正常胃黏膜组织及癌组织的病理结构;采用免疫组织化学方法检测SIRT-1和E-cadherin在正常胃黏膜组织及癌组织中的表达水平;Spearman相关检验检测SIRT-1与E-cadherin表达的相关性.结果:HE染色结果:胃癌组织中癌细胞分化差,多形性,大小不一,可呈假复层,核大,胞浆少,容易找到核分裂.免疫组织化学检测结果:SIRT-1在正常胃黏膜组织和胃癌组织的阳性表达率分别为40.5%和64.3%;E-cadherin的阳性表达率分别为73.8%和40.5%.两组SIRT-1和E-cadherin的阳性表达率差异有统计学意义(P<0.05).Spearman相关分析显示SIRT-1与E-cadherin的表达水平呈负相关(P<0.05).胃癌组织中SIRT-1蛋白阳性表达与肿瘤大小及病理类型有关(P<0.05);而与患者的年龄、性别、TNM分期及淋巴结转移无关(P>0.05).结论:胃癌组织中SIRT-1高表达可能通过抑制E-cadherin促使肿瘤组织细胞发生侵袭、转移.%Objective:To research the expressions and relationship of silence signal regulating factor-1(SIRT-1)and epithelial cadherin(E -cadherin)in gastric cancer. Methods:HE staining was used to observe the his-topathologic structure in the cancer group and normal gastric mucosa group.Immunohistochemistry SP technique was used to detect the expressions of SIRT -1 and E - cadherin in cancer group and normal gastric mucosa group. Spearman correlation test was used to detect the expression correlation of SIRT-1 and E-cadherin.Results:HE staining gastric cancer tissue:Cell differentiation was poor,pleomorphism and different size.Cells showed fake many layers,cell nucleus was large and karyokinesis,kytoplasm was few.The immunohistochemistry showed the positive ex-pression of SIRT-1 were 40.5% and 64.3% in the normal gastric mucosa group and cancer group.The positive ex-pression rate of E-cadherin were 73.8% and 40.5% respectively in the normal gastric mucosa group and cancer group(P<0.05).Spearman correlation test showed that there was a correlation between SIRT-1 and E-cadherin (P<0.05).SIRT-1 protein expression was related to the tumor size and pathological types(P<0.05).But not re-lated to the age,gender,TNM stage and lymph node metastasis(P>0.05).Conclusion:Gastric cancer with the high-er SIRT-1 expression may be the ways to achieve tumor invasion and metastasis through E-cadherin as a facilita-tor.