恒河猴睾丸支持细胞对间质细胞分泌睾酮的研究

摘要

目的 研究恒河猴睾丸支持细胞对间质细胞分泌睾酮的调节作用.方法 采用无血清培养的方法,分析了促性腺激素释放激素(GnRH)、卵泡刺激素(FSH)、表皮生长因子(EGF)、内皮素-1(ET-1)和白细胞介素-1(IL-1)对原代共培养恒河猴睾丸支持细胞与间质细胞睾酮分泌水平的影响.结果 GnRH、FSH、EGF和ET -1对原代共培养恒河猴睾丸支持细胞与间质细胞睾酮分泌水平具有促进作用,并且这种影响与共培养的支持细胞数量呈线性关系,即共培养的支持细胞数量增加,睾酮分泌水平亦明显上升；而IL-1对原代共培养恒河猴睾丸支持细胞与间质细胞睾酮分泌水平没有产生影响.结论 支持细胞对间质细胞分泌睾酮具有重要的调节作用.%Objective To study the regulation of the testosterone secretion of leydig cells from sertoli cells of the rhesus monkey. Methods By using serum - free, we analyzed that the effect of gonadotropin - releasing hormone (GnRH), follicle stimulating hormone ( FSH ) , epidermal growth factor ( EGF) , endothelin 1 ( ET - 1) and interleukin - 1 (IL - 1) on the secretion of testosterone in sertoli -leydig co - culture. Results The experimental results showed that GnRH, FSH, EGF and ET - 1 stimulated the testosterone secretion of sertoli - leydig co - culture, and the relation between this influence and the number of sertoli cells cultivation was linear, as that the increase of sertoli cells would lead to significantly raise of the testosterone secreation; but the IL - 1 would not affect testosterone secretion for sertoli - leydig co - culture. Conclusion These results suggest that the key role of steroli cells in the regulation of leydig cells to secrete testosterone.