目的 探讨丙泊酚对肠缺血再灌注损伤的保护作用及其可能的机制.方法 24只大鼠随机分为3组:假手术组(S组)、缺血再灌注+生理盐水组(I/R+NS组)、缺血再灌注+丙泊酚组(I/R+P组),各组8只.夹闭肠系膜上动脉,缺血1 min,再灌注2h制备小肠缺血再灌注模型.S组和I/R+NS组于术前10 min开始分别静脉泵注生理盐水10 mL/(kg·h),(I/R+P)组于夹闭肠系膜上动脉前10 min静脉泵注丙泊酚10 mg/(kg·h).分别测定各组动物血浆、肠组织肿瘤坏死因子-α(TNF-α)含量,光学显微镜观察肠黏膜损伤情况.结果 光学显微镜观察组织损伤:I/R+P组肠黏膜损伤程度减轻,与I/R+NS组比较有显著性差异(P＜0.01).I/R+P组血浆及肠组织TNF-α的水平降低,与I/R+ NS组比较有显著性差异(P＜0.01).结论 丙泊酚可明显减轻大鼠肠缺血再灌注导致的肠黏膜损伤,这种作用可能是通过抑制TNF-α的释放实现的.%Objective To study the potential role and possible protective effect of propofol on tumor necrosis factor-a (TNF-α) release after intestinal ischemia-reperfusion. Methods' Twenty four rats were randomly divided into three groups: sham-operated control group (S, n=8), ischemia-reperfusion+NS group (I/R+NS, n=8) and ischemia-reperfusion+propofol group (I/R+P, n=8). Ischemic reperfusion models were made by occluding the superior mesenteric artery for 1 minute, followed by 120 minutes reperfusion. S and I/R+NS group were pretreated with sodium chloride 10 mL/(kg-h) before operation, while I/R+P were pretreated with propofol 10 mg/(kg.h) before occlusion. The expression levels of TNF-a in the small intestinal tissue and plasma were evaluated by enzyme-linked immunosorbent assay (ELISA) and the condition of mucous membrane lesion was observed under optional microscope in each group. Results Propofol could availably alleviate the pathologic lesion of small intestine mucosa caused by intestinal ischemia-reperfusion and could decrease the elevation of plasma and small intestinal tissueTNF-α levels after intestinal ischemia-reperfusion. Conclusion Propofol can reduce the injury of ischemia-reperfusion in rat. Such protective effect may be achieved through the inhibition of TNF-α release.
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