基于光动力学姜黄素水凝胶治疗痤疮的疗效评价

摘要

目的 证明姜黄素水凝胶结合光动力学治疗痤疮的效果.方法 采用过200目筛、超声/高压均质技术使姜黄素微粉化.以泊洛沙姆407为基质,制备姜黄素水凝胶并对所制备的水凝胶进行流变学及流体学表征.采用打孔法考察姜黄素水凝胶在657 nm激光照射下的体外抑菌效果.建立兔耳痤疮模型后将15只新西兰白兔随机分为5组,分别为模型组、维A酸乳膏组、单独光照组、姜黄素水凝胶组、姜黄素水凝胶/光照组,治疗2周后肉眼观察兔耳外观并进行组织病理学检测.结果超声/高压均质法制备的微粉化姜黄素粒径更小、更均一,粒径范围为5~20 μm;过筛法制备的微粉化姜黄素粒径较大,分布不均,粒径范围为10~45 μm.所制备的水凝胶具备凝胶的特性,较稳定,为假塑性流体.姜黄素水凝胶/光照组的体外抑菌效果优于姜黄素水凝胶组,姜黄素水凝胶组、姜黄素水凝胶/光照组的抑菌圈直径大小分别为(9.4±0.92)和(12.57±1.12)mm.在兔耳痤疮模型上验证了姜黄素水凝胶/光照对兔耳痤疮的治疗效果优于单独光照组和姜黄素水凝胶组.结论 姜黄素水凝胶结合光动力学对兔耳痤疮有一定治疗效果,为痤疮治疗提供了一种新选择.%Objective To demonstrate the therapeutic effect of curcumin hydrogel combined with photodynamics on acne. Methods Sieving and ultrasound/high pressure homogenization were used to prepare curcumin micropowder.Curcumin-loaded hydro?gel was prepared with Poloxamer 407 and its rheological characteristics were evaluated.The antibacterial activity of curcumin hydrogel irritated with 657 nm laser was assayed by a hole-punching method.Acne model on rabbit ears war established.Fifteen New Zealand white rabbits were randomly divided into five groups:the model group,tretinoin cream group,illumination group,curcumin hydrogel group,and curcumin hydrogel/illumination group.The therapeutic effects on acne were evaluated by the morphological and histopatho?logical examination.Results The micropowdered curcumin prepared with ultrasound/high pressure homogenization had smaller size and more uniform distribution(size range:5-20 μm.)than the sieving-prepared curcumin micro-powders(10-45 μm).The prepared hydrogels being a pseudoplastic fluid had the properties of gelatin.The antibacterial effect of curcumin hydrogel/illumination was bet?ter than that of curcumin hydrogel.The inhibition zone diameters were(9.4±0.92)and(12.57±1.12)mm for the curcumin hydrogel and the curcumin hadrogel/illumination groups,respectively.The therapeutic effect for the treatment of acne on the rabbit ears with ac?ne was better in the curcumin hydrogel/illumination group than in both the illumination and the curcumin hydrogel groups. Conclu?sion Curcumin hydrogel combined with photodynamic therapy showed better therapeutic efficacy on acne treatment.It provides an ideal choice for acne treatment.