低聚原花青素对肉鸡生长性能、免疫机能及抗氧化性能的影响

摘要

[Objective]This experiment was conducted to evaluate the effects of OPC on growth per-formance,immune function and antioxidative induces in serum of broiler chick aged from 1 to 42 days.[Method]200 healthy male broilers (AA)at 1-day-old were randomly allotted into 4 groups with 5 repli- cate in each group,and 10 broiler chicks per replicate.Chicks were fed a basal diet supplemented with 0,5, 10,15 mg/kg OPC.The experiment lasted for 42 days.We measured growth performance indexes (ADG, ADFI and F/G),antioxidation indexes of plasma (T-AOC,GSH-Px,T-SOD and MDA),and immune inde-xes (immune organ index,activity of mononuclear phagocytic cells and lysozyme level in plasma).[Result]1)5 mg/kg and 10 mg/kg OPC significantly increased ADG during 22-42 days(P<0.05).ADFI and F/G decreased during 22-42 days in each group(P<0.05).5 mg/kg and 10 mg/kg OPC significantly reduced ADFI and F/G during 1-42 days.2)10 mg OPC significantly increased T-AOC in Serum at 21 days(P<0.05).GSH-Px,T-SOD,T-AOC increased significantly in 10 mg OPC and 15 mg OPC groups at 42 days(P<0.05).3)Thymus index of broiler significantly increased in each group at 42 days(P<0.05),and Spleen index significantly increased in 10 mg/kg OPC group(P<0.05).4)Clearance index significantly increased in 10 mg/kg and 15 mg/kg OPC group(P<0.05),and phagocytic index significantly increased in 10 mg/kg OPC group(P<0.05).5)5 mg/kg OPC and 10mg/kg OPC improved lysozyme activity in serum.[Conclu-sion]Diet supplemented with 10mg/kg OPC can significantly improve growth performance and immune pa-rameter,15mg/kg OPC improves serum antioxidant index mostly.%[目的]研究低聚原花青素(OPC)对肉鸡生长性能、免疫机能及抗氧化性能的影响.[方法]选取 1 日龄艾拔益加(AA)肉鸡(公雏)200 只随机分为 4 个组,每组 5 个重复,每个重复 10 只鸡.分别饲喂添加 0,5,10,15 mg/kg OPC的饲粮,进行为期42 d的试验.测定了平均日增质量、平均日采食量、料重比等生长性能指标,总抗氧化能力(T-AOC)、谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(T-SOD)、丙二醛(MDA)等血浆抗氧化指标以及免疫器官指数、单核吞噬细胞活性、血浆溶菌酶等免疫指标.[结果]1)5、10 mg/kg OPC可显著提高 22~42 日龄平均日增质量(ADG)(P<0.05),以 10 mg/kg OPC 组最高,显著降低平均日采食量(ADFI)及料重比(F/G) (P<0.05),以 10 mg/kg OPC 组最低;5、10 mg/kg OPC 均可显著降低 1~42 日龄 ADFI、F/G(P<0.05),以10 mg/kg OPC组最低;2)10 mg/kg OPC组可显著提高 21 d血浆总抗氧化能力(T-AOC);10、15 mg/kg OPC可显著提高42 d 谷胱甘肽过氧化物酶(GSH-Px)水平、超氧化物歧化酶(T-SOD)水平、T-AOC水平;3)各 OPC剂量均可显著提高肉鸡胸腺指数水平(P<0.05),且1 0 mg/kg OPC 组优于1 5 mg/kg OPC组.1 0 mg/kg OPC可显著提高脾脏指数水平(P<0.05);4)10、15 mg/kg OPC组可显著提高廓清指数水平(P<0.05),且 10 mg/kg OPC 组优于 1 5 mg/kg OPC组.1 0 mg/kg OPC组可显著提高吞噬指数水平(P<0.05 );5 )5 、1 0 mg/kg OPC 组均可显著提高血浆溶菌酶含量(P<0.05),以 10 mg/kg OPC组最优.[结论]10 mg/kg OPC 对肉鸡 1~42 d 的生长性能及免疫机能提升最显著,1 5 mg/kg OPC对肉鸡血浆抗氧化提升最显著.