To investigate the influence of bensulfuron-methyl(BSM)on culturable microbial quantities and unculturable microbial community structures,conventional and molecular biological methods were employed in five BSM treated soils with three replications,respectively. The results obtained with traditional culture-dependent methods showed that a low-level of BSM had slight and transient effects on culturable microorganisms;nevertheless,high concentration of BSM resulted in a dramatic decrease in bacterial colony forming units (cfus).The result obtained using denaturing gradient gel electrophoresis(DGGE)revealed that more than 17 bands were observed in low BSM contaminated soil samples and only 10 bands were detected in samples with high BSM contamination.In other words,the diversity of soil community structure is related to the concentration of BSM.Cluster analysis showed that the community structure under low level of contamination was more similar to that of the control,while heavy contaminated amendments were far away from the above group.In a sense,the cooperation of the traditional method and the molecular biological method is more powerful to study the soil microbial information in contaminated ecosystem.
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机译:分子生物技术在污泥微生物群落多样性研究中的应用—污泥微生物群落研究 Application in Activated Sludge Microbial Community Diversity Used Molecular Biological Technology—Study of Sludge Microbial Community Diversity
机译:Intra- and Inter-annual Variation of Soil Microbial and Enzymatic Response to Water and Nitrogen Addition in a Chinese Semi-arid SteppeAU Sun, Liangjie Dong, Yunshe Qi, Yuchun (qiyc@igsnrr.ac.cn) He, Yating Peng, Qin Liu, Xinchao Jia, Junqiang Guo, Shufang Cao, Congcong