Estrogenic effects of water from the Yangtze River (Nanjing section) on goldfish (Carassius auratus) after an early life stage exposure

摘要

Effects of river water from the Yangtze River (Nanjing section) on fish development, vitellogenin (VTG) induction, gonado-somatic index (GSI) and sex ratio were investigated by exposing goldfish (Carassius auratus) in the early life stage (from fertilization to 28 days post-hatch) to water samples (25%, 50% and 100%) collected from three representative sections. The results showed that there was no significant effect on hatching success for any of the exposure groups, but survival was significantly reduced when compared with the control (P < 0.05). Body lengths, weights of all treated fish did not differ significantly from those of the control. Condition factors (CF) of larval fish exposed to 50% and 100% river water from the Jiangxinzhou section and 100% river water from the Daqiao section were significantly lower than that of the control (P< 0.05). VTG inductions were significant in larval fish exposed to all the dilution series of river water. No significant difference in CF value was observed in any exposure group after 150 days of depuration. VTG was fully eliminated after 75 days of depuration. For both female and male, GSI did not significantly differ between exposure groups and the control after 150 days of convalescence. The highest female:male ratios were observed in response to the treatment with 50% or 100% river water from the Jiangxinzhou section and 100% river water from the Daqiao section (53:47, 56:44 and 54:46, respectively), but no significant difference in sex ratio was observed in any treated group when compared to the control. The results showed that early life stage exposure of river water from the Yangtze River (Nanjing section) had adverse effects on goldfish development and reproductive health, and the effects on CF and VTG were reversible after depuration in clean water.