Objectives The intestinal flora of healthy term infants delivered by cesarean section was analyzed by means of quantitative real-time polymerase chain reaction assays and the effect of formula-feeding and breast-feeding on the development of gut microflora was investigated. Methods Sixty healthy term infants born by cesarean section delivery were enrolled in this study and divided into two groups: breast-fed group (n= 30) and formula-fed group (n=30). Anthropometric measurements, questionnaire about stool characteristics and stool samples were taken at enrollment time of 6 weeks after birth. Stool samples were subjected to quantitative real-time polymerse chain reaction assays for determination of lactobacili, bifidobacteria, Bifidobacterium longum, Bifidobacterium breve and Bifidobacterium adolescenitis. Results The frequency and water content of stool in breast feeding infants were higher than in formula feeding infants. The real-time PCR showed that the intestinal microbiota of breast-fed infants had higher numbers of bifidobacterium compared with that of formula-fed infants (P ＜ 0.05). The number of B.longum had no significant difference between two groups, however, was higher in the breast-fed infants. Conclusions The amount of bifidobacterium in the intestine of breast-fed infants are higher than that of formula-fed infants.%目的 研究纯母乳喂养或纯配方奶喂养组对婴儿大便性状及肠道菌群的影响.方法 以健康足月剖宫产婴儿为观察对象,母乳喂养组和配方奶喂养组婴儿各30例.在婴儿6周龄时采集婴儿粪便标本,同时记录婴儿体质量、头围及大便情况.应用实时荧光定量PCR技术对肠道乳酸杆菌属、双歧杆菌属,以及双歧杆菌的某些重要亚种如长双歧等,进行定量检测.结果 母乳喂养婴儿的大便次数及大便含水量明显高于配方奶喂养婴儿.母乳喂养婴儿的双歧杆菌数量明显高于配方奶喂养婴儿(P ＜ 0.05);长双歧数量母乳喂养婴儿高于配方奶喂养婴儿,但两组间差异无统计学意义.结论 剖宫产母乳喂养儿的肠道双歧杆菌数量明显高于配方奶喂养儿.
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