Objective To evaluate the diagnostic value dynamically of fluorescent quantization PCR (FQ-PCR) forMP-DNA, enzyme-linked immunosorbent assay (ELISA) for MP-IgM antibodies, and rapid culture for MP in children with Mycoplasma pneumoniae infection. Methods The pediatric patients with confirmed MP infection by clinical and laboratory diagnosis were tested for MP-DNA, MP-IgM, and MP at admission, one month and three months after admission. Results A total of 153 MP infected pediatric patients were enrolled. At admission, the detection rate of FQ-PCR, ELISA and MP rapid culture were 96% , 71% and 24% respectively. The detection rate of rapid culture method was significantly higher in < 3 years old than that in > 3 years old group (P < 0.05). At one month, the detection rate of MP-DNA was > 50%, and the detection rate of MP-lgM was still higher. Forty-four patients (29%) had cough at one month, among whom 28 (64%) patients were both MP-DNA and MP-IgM positive. At 3 months, 4 (3%) patients had cough, among whom 2 patients were both MP-DNA and MP-IgM positive. Conclusions In three kinds of methods, FQ-PCR assay for detecting MP-DNA was the most sensitive method for diagnosing MP infection, followed by the ELISA assay for detecting MP-IgM and rapid culture for MP. The detection rate of MP-IgM remained a high level when follow-up to 3 months.%目的 评价荧光定量PCR (FQ-PCR)检测肺炎支原体(MP)-DNA(MP-DNA),酶联免疫吸附试验(ELISA)检测MP-IgM,以及MP快速培养三种方法对儿童MP感染诊断的临床价值.方法 对经临床和实验室诊断MP感染的患儿同时进行三种方法检测.并分别在病程1、3个月时重复检测.结果 MP感染患儿共153例.入院时首次检测,FQ-PCR阳性率为96％,ELISA为71％,MP快速培养法为24％.其中MP快速培养法的检出率在≤3岁组明显高于＞3岁组,差异有统计学意义(P＜0.05).随访至1个月时,MP-DNA检测结果仍有50％以上阳性,而MP-IgM则保持较高的阳性率,此时仍然有44例(29％)患儿存在咳嗽,其中28例(64％)MP-DNA和MP-IgM均为阳性.随访至3个月时有4例(3％)患儿仍存在咳嗽,2例(50％)MP-DNA和MP-IgM均为阳性.结论 在三种诊断儿童MP感染的方法中,FQ-PCR检测MP-DNA最敏感,其次为ELISA检测MP-IgM抗体,MP快速培养敏感性最低.MP-IgM检测阳性率至3个月时仍然维持较高水平.
展开▼
