线粒体在宫内生长受限胎盘中的变化

摘要

Objective To explore the mechanism of intrauterine growth restriction (IUGR) via observing the change of mitochondria in IUGR placenta. Methods Placenta samples were collected from 30 singleton pregnancies at the time of elec-tive caesarean section. Fifteen of them were appropriate for gestational age and 15 were IUGR. Mitochondrial morphology was observed by transmission electron microscopy, DNA copies were analyzed by real-time quantitative PCR and membrane potential was assayed by lfow cytometry. Results Signiifcant morphological changes of placental mitochondria were observed under transmission electron microscopy in IUGR, mitochondrial DNA copies in IUGR placenta were signiifcantly increased (P<0.01) and membrane potential decreased dramatically (P<0.01). Conclusions It is suggest that impaired mitochondrial function in IUGR may involve in IUGR pathogenesis.%目的：观察宫内生长受限（IUGR）胎盘线粒体的变化，探讨IUGR机制。方法胎盘样本取自30例选择性剖宫产的单胎妊娠产妇，15例为适于胎龄儿（AGA），15例为IUGR。采用透射电子显微镜观察胎盘线粒体形态，实时定量PCR分析线粒体DNA拷贝数，流式细胞术检测线粒体膜电位。结果透射电子显微镜下显示IUGR胎盘的线粒体形态明显改变；线粒体DNA拷贝数显著上调（P<0.01）；而线粒体膜电位明显下降（P<0.01）。结论 IUGR胎盘中线粒体功能明显受损，可能参与IUGR的发生。