母乳中烷氧基甘油含量的测定

摘要

Objective To determine alkylglycerol (AKG) contents and variation in breast-milk of lactating women. Methods Five cases of healthy lactating women with term delivery were selected from June 2011 to June 2012. Breast-milk samples were collected at 1, 2, 3, 4, 8, 12, 16, 20 and 24 weeks postpartum. Breast milk samples were extracted, saponificated and derivatized. AKGs composition in breast-milk was quantitatively analyzed by GC chromatography. Results Mean 16C:0 AKG content in breast-milk decreased from(17.31 ± 3.59)× 10-3g/L to(11.14 ± 1.83)× 10-3g/L. Mean 18C:0 AKG content de-creased from(14.95±6.00)×10-3g/L to(9.68±2.51)×10-3g/L. Mean 18C:1 AKG content fluctuated between(4.64±0.91)×10-3g/L and(3.95±0.68)×10-3g/L. Conclusions 16C:0, 18C:0 and 18C:1 AKG contents exist in Chinese breast-milk through determina-tion by GC chromatography, and the concentrations vary among different stages of lactation.%目的：探讨哺乳期妇女乳汁中烷氧基甘油（AKGs）含量及其变化规律。方法选取2011年6月至2012年6月足月单胎分娩的健康产妇5例，收集哺乳期第1、2、3、4、8、12、16、20、24周母乳用于AKGs测定。母乳样品经萃取、皂化、提取、衍生化处理后，通过气质联用色谱内标法对母乳中AKGs成分进行定量分析。结果母乳中16C∶0 AKG含量逐渐降低，均值从（17.31±3.59）×10-3g/L降低到（11.14±1.83）×10-3g/L，而18C∶0 AKG均值从（14.95±6.00）×10-3g/L下降到（9.68±2.51）×10-3g/L，18C∶1 AKG均值在（4.64±0.91）×10-3g/L到（3.95±0.68）×10-3g/L之间。结论通过气质联用色谱内标法检测到哺乳妇女母乳中存在16C∶0、18C∶0和18C∶1三种AKG，其浓度在哺乳期不同阶段有所改变。