Gelatin microspheres were made by improved emulsified cold condensation method. Effects of TGF-β1 gelatin microspheres on the proliferation and differentiation of MG63 cells were investigated in vitro, as well as the optimal TGF-β1 concentration. Porous titanium with a porosity of 60% and an inter-connected structure was prepared using a powder injection molding method. A coating of TGF-β1 loaded gelatin microspheres was applied in the pores of the porous titanium surface. MTT assay method was used to evaluate the cytotoxicity of the coating to L929 cells. The pharmacokinetics of the coating was tested with TGF-β1 ELISA kit. In vitro, the effect of the coating on adhesion, proliferation and differentiation of MG63 cells was investigated. The results show that the TGF-β1 concentration affects the function of MG63 cells. In vitro, the proliferation and differentiation of MG63 cells show a positive dose-effect relationship at TGF-β1 concentrations of 0.025-2.500 mg/g. TGF-β1, concentration of 2.500 mg/g is in favor of the differentiation, while the concentration of 25 mg/g inhibites cells' proliferation. Porous titanium samples coated with TGF-β1 gelatin microspheres are nontoxic to L929 cells and are able to maintain a slow-release state during 12 d. The porous titanium implants coated with TGF-β1 loaded gelatin microspheres benefits MG63 cells adhesion, proliferation and differentiation on the 3th, 7th, 14th slot.%用改良乳化冷凝聚合交联法制备明胶缓释微球,体外评价载TGF-β1明胶微球对MG63细胞增殖和分化的影响,并优化TGF-β1作用浓度;用粉末注射成形技术制备60％孔隙度具有连通孔结构多孔钛,用涂覆法在多孔钛表层孔隙内制备载TGF-β1明胶微球涂层,MTT法检测该涂层对L929细胞毒性,ELISA法检测该涂层的药物动力特性,体外评价该涂层对MG63细胞的黏附、增殖和分化的影响.研究结果表明:TGF-β1浓度影响MG63细胞功能,在0.025～2.500 mg/g范围内,TGF-β1对MG63细胞的增殖和分化作用呈剂量正效应关系,浓度为2.5mg/g时,对MG63细胞分化作用最优,而TGF-β1浓度为25 mg/g时,对MG63细胞增殖有抑制作用；载TGF-β1明胶微球涂层多孔钛无细胞毒性,涂层具有缓释功能,能维持12d,该涂层有利于MG63细胞的黏附、增殖和分化.
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