为了探索不同外源激素对油桐花芽分化过程中关键成花基因表达的影响，采用不同种类、不同浓度梯度的外源激素在未分化期进行叶片喷施处理，分析研究对油桐成花基因Tunglfy基因表达量的差异，以期为油桐花期调控的分子生物学研究提供支撑和依据。结果表明：外施6-苄基腺嘌呤对Tunglfy基因表达均有抑制作用，整个花芽分化进程中较对照都降低了Tunglfy基因表达量。不同浓度赤霉素对Tunglfy基因的表达有着不同的影响，高浓度赤霉素对Tunglfy基因的表达具有抑制作用，而低浓度则起到促进作用。脱落酸的50 mg/L和200 mg/L对Tunglfy基因的表达有较强的抑制作用，而100 mg/L对Tunglfy基因的表达均有明显的促进作用。%In order to explore different exogenous hormones on key flowering genes expression in Aleurites fordii flower bud differentiation process, the leaves spraying treatments were conducted by adopting exogenous hormones with different types and concentrations in the undifferentiated stage, and the differences of exogenous hormones on flowering genes expression levels of Tunglfy genes were investigated, thus providing support and basis for molecular biology research of control the flowering phase. The results show that the applying 6-benzyladenine to the buds had inhibitory action on Tunglfy gene expression, in the entire process of flower bud differentiation, the treated lowered down more the amount of Tunglfy gene expression than the control; There were differences among different concentrations of gibberellic acid on Tunglfy gene expressions, the high concentrations of gibberellic acid on Tunglfy had inhibitory effect to Tunglfy gene expression, while the low concentrations played a facilitating role. The treatments with abscisic acid of 50 mg/L and 200 mg/L had strong inhibition to Tunglfy gene expression, and 100 mg/L for Tunglfy gene expression had obvious promoting role.
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