Wolbachia对苹果蠹蛾生殖调控的作用

摘要

Background] Codling moth ( Cydia pomonella) is the most serious fruit tree and quarantine pest in Chinese orchards. The species is host to Wolbachia, Dor subgroup of the A group, which induce cytoplasmic incompatibility. The aim of this study is to find out if the Wolbachia induce cytoplasmic incompatibility to reproductive regulation in C. pomonella. [Method] The relative fit￣ness of C. pomonella with active Wolbachia was compared to individuals in which Wolbachia was eliminate by antibiotics. [Result]We found no significant differences in development duration, fecundity, hatchability and pupal survival of the F1 , and F2 generations between the two groups. Wolbachia infection had no effect on adult life span, the pre￣oviposition period, fecundity and female ratio in C. pomonella. Hybridization tests showed that hybrids could lay eggs, and these eggs were normal. [Conclusion and significance]Wolbachia in C. pomonella did not induce host cytoplasmic incompatibility.%【背景】苹果蠹蛾是重要的果树害虫和检疫对象。研究显示,苹果蠹蛾体内所含的Wolbachia属于具有诱导胞质不亲和( CI)现象的A组中Dor亚组。本研究目的在于摸清苹果蠹蛾体内的Wolbachia对宿主的生殖调控是否具有诱导胞质不亲和的功能。【方法】通过饲喂抗生素消除苹果蠹蛾体内的Wolbachia,比较感染与消除Wolbachia的苹果蠹蛾生态适合度以及Wolbachia感染对苹果蠹蛾生殖的影响。【结果】消除Wolbachia后,苹果蠹蛾F1、F2代各虫态的发育历期、产卵量、孵化率和蛹的存活率与对照无显著差异；Wolbachia对苹果蠹蛾成虫的寿命、产卵前期、平均产卵量和性别比例均无影响。不同处理苹果蠹蛾之间的杂交试验结果表明,4种杂交的苹果蠹蛾都能产卵,且卵可以正常孵化。【结论与意义】在自然状态下苹果蠹蛾体内的Wolbachia不能诱导其宿主的胞质不亲和现象。