The joint toxicity of heavy mental ions copper, lead , cadmium and zinc to Moina macrocopa was evaluated basing on acute toxicity of the four kinds of single-factor by using marking additive index method. The result showed that the toxicity sequence of four heavy metals to Moina macrocopa was Cd2+ ＞ Cu2+ ＞ Pb2+ ＞ Zn2+ . The median lethal concentrations( LC50 ) of 48h for copper, lead,cadmium and zinc to Moina macrocopa were 0. 0076mg/L, 0. 0306mg/L, 0. 0532mg/L and 0. 3568mg/L, respectively. The toxicity of mixture Pb2+ - Cu2+ - Cd2+ , Pb2+ - Zn2+ - Cd2+ and Cu2+ - Pb2+ - Cd2+ - Zn2+ showed to be antagonistic. The joint toxicity of Cu2+ - Zn2+ - Cd2+ and Pb2+ - Cu2+ - Zn2+ was complicated. When the concentration proportion of the three was 1 ∶1 ∶1 , they were synergetic to each other; however. if their toxicity proportion was 1 ∶1 ∶1, they were synergetic at 24h, but antagonistic at 48h.%在研究了铜、铅、镉和锌4种单一因子对多刺裸腹溞(Moina macrocopa)急性毒性作用基础上,采用Marking 相加指数法研究了联合毒性效应.实验结果表明:4种重金属离子的毒性大小依次为Cd2+＞Cu2+＞Pb2+＞Zn2+,对多刺裸腹溞48h的LC50分别为0.0076mg/L,0.0306mg/L,0.0532mg/L,0.3568mg/L;Pb2+-Cu2+-Cd2+、Pb2+-Zn2+-Cd2+3种离子和Cu2+-Pb2+-cd2+-Zn2+4种离子共存时的毒性效应表现为拮抗作用;Cu2+-Zn2+-Cd2+和Pb2+-cu2+-Zn2+共存时的联合毒性较为复杂,三者浓度比1:1:1时表现为协同作用,而毒性比1∶1:1时在24h内表现为协同作用,至48h时为拮抗作用.
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