化州柚查尔酮合成酶基因克隆与序列分析

摘要

To clone chalcone synthase ( CHS) gene from the Citrus grandis tomentosa, which was the genuine medicinal materials from Guangdong province, CTAB-LiCl treatment was used to extract RNA from Citrus grandis tomentosa' and RT-PCR was used to clone the CHS gene at the same time. The CHS gene included the open reading frame( ORF) , and it was consisted of 1173bp nucleic acid, encoded 391 amino acid residues. Compared with other CHS genes from citrus plant, it was found that they shared a sequence homology more than 98％ at the nucleic acid level. Result showed that CTAB-LiCl treatment could extract high quality RNA from Citrus grandis tomentosa'. The cloned CHS gene from Citrus grandis tomentosa' had the open reading frame and shared a high sequence homology with other Citrus plant.%利用CTAB-LiCl法提取高质量的化州柚总RNA,采用RT-PCR技术克隆查尔酮合成酶基因,获得广东道地药材化橘红资源化州柚的查尔酮合成酶基因.该基因编码区全长1176bp,编码391个氨基酸残基,与同样来源于柑橘属的查尔酮合成酶基因同源性高达98%.CTAB-LiCl法能提取高质量的化州柚总RNA,可以用于后续基因克隆和分析;克隆获得的查尔酮合成酶具有编码区,与同属植物相同基因具有高度序列同源性.