菖蒲过氧化物酶测定条件的研究

摘要

Peroxidase is a key enzyme in plant resistant system for adversities such as low temperature. This article studied the effect of different determination systems, H2O2 concentration, pH of the buffer and reaction time on the peroxidase activity in Acorus calamus. The optimal measure condition is 3 mL for reaction system, 0.1% for H2O2 concentration, 6.0 for pH of the buffer and 4min for reaction time. The obtained conclusions provided with referential value for the selection and valuation of hardy plants in constructed wetlands and plants physiological experimental teaching and research.%过氧化物酶是植物在低温等逆境条件下酶促防御系统的关键酶之一,其活性可以作为植物抗寒性的一个指标、以菖蒲为材料比较了反应体系、H2O2浓度、pH值及反应时间对过氧化物酶活性测定的影响 结果表明:过氧化物活性测定的较佳反应体系是3 mL；最佳H2O2浓度为0.1％;最佳磷酸缓冲液pH值为6.0；最佳反应时间为4min.该研究结果为人工湿地污水处理系统冬季植物的筛选与评价研究提供参考,对植物生理实验教学和相关科学研究有一定的参考价值.