苏姜猪及其杂交后代 HSL 基因 PCR-RFLP 分析

摘要

以苏姜猪、二元杂交猪（长白猪×苏姜猪、大白猪×苏姜猪）为研究对象，分别为60头，分析了HSL基因外显子Ⅰ区域PCR-RFLP多态性。结果表明，苏姜猪及其杂交后代群体中HSL基因外显子I中存在Bsa H I酶切片段长度多态性，均检测到AA、AB和BB基因型。苏姜猪群体中A等位基因占优势，频率为73.34％；杂交猪中B等位基因占优势，频率分别为56.66％和65.00％。研究结果丰富了苏姜猪的分子生物学研究，为苏姜猪的合理开发利用提供了依据。%The polymorphism of HSL gene in swine was studied using PCR-RFLP.Total 180 pigs were selected in this experiment, which were Sujiang pig and it′s crossbreed with Large White and Landrace, 60 in each grow.The polymorphism of Bsa H I in HSL gene exon I was detected by restriction fragment length polymorphism (RFLP) method.Three genotypes of AA, AB and BB were de-tected in three pig populations. It showed that Aallele was dominant in Sujiang pig groups with 73.34%, and B allele was detected mainly from the crossbreed with 56.66%and 65.00%, respectively. The results will enrich Sujiang pig molecular biology research, and provide the basis for the reasonable development and application of Sujiang pig.