以大决明子为原材料，基于氨基酸比值系数法，对决明子的营养价值进行了分析；并以碱性缓冲液为提取剂，进行决明子蛋白提取工艺研究，探讨了缓冲液浓度及pH值、料液比、浸泡时间对蛋白提取率的影响，最后用正交试验确定大决明子蛋白的最佳提取工艺。结果表明决明子的营养价值高于大豆和紫花苜蓿，与南瓜接近略低于鸡蛋；提取工艺条件最佳为50 mmol/L、pH值＝8.0的KH2PO4-NaOH缓冲液，料液比1 g：50 mL，浸泡提取时间12 h。此条件下决明子蛋白的提取率为93.3％。%Based on the essential amino acid index , the nutritive value of Semen cassiae was analyzed to be higher than those of soya bean and alfalfa , which was approached to that of pumpkin and lower than that of egg .The effects of alkaline buffer concentration , al-kaline buffer pH, sample-water ratio, the extracting time on Semen cassiae protein extraction ratio were investigated by using buffer so-lution as extracting reagent .The optimal conditions for extracting protein from Semen cassiae were confirmed through orthogonal test , and the results were as follow:the alkaline buffer concentration , 50 mmol/L;pH 8.0, the ratio of sample to water, 1∶50 (g/mL);the extracting time, 12 h.Under the condition, the extraction rate of Semen cassiae protein was 93.3%.
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