黄酮3-羟化酶(F3H)的生物信息学分析

摘要

通过生物信息学分析方法,对已经在GenBank上注册的青稞( Hordeum vulgare)、高粱( Sorghum bicolor)、小麦( Triticum aestivum)、玉米( Zea mays)、洋葱( Allium cepa)、花烛( Anthurium andraeanum)和美丽百合( Lilium specio-sum)等7种单子叶植物的F3H核酸以及相应氨基酸序列进行研究,并对其组成成分、理化性质、信号肽、亚细胞定位、跨膜结构、蛋白质二级、三级结构、结构域以及分子进化等方面进行分析与预测. 研究结果表明,除玉米F3 H蛋白定位于过氧化物酶体外,其他植物F3 H均定位于细胞质基质内;所有植物F3 H均为不含信号肽的非分泌型蛋白,且没有跨膜区域的亲水性蛋白;α-螺旋和无规则卷曲是F3 H的主要二级结构元件,而延伸链则散布于整个蛋白中;F3H拥有一个2OG-Fell Oxy super family和一个PLNO3176 super family保守结构域;所构建的F3H系统发育进化关系与形态学上物种发育关系基本吻合.%In this paper, F3H, sequences from Hordeum vulgare, Sorghum bicolor, Triticum aestivum, Zea mays, Allium cepa, An-thurium andraeanum and Lilium speciosum, which were registered in GenBank, were analyzed and predicted by tools of bioinformatics in the following aspects:composition, physical and chemical properties, signal peptide,protein subcellular localization, transmembrane helices in proteins, structure of protein, conserved domain and phylogeny. The results demonstrated Zea mays F3H lies in peroxisome and F3H from other plants locate in cell matrix;F3H is a non-secretory protein without signal peptide and hydrophilic protein without transmembrane area;α-helix and random coil are primary secondary structural components of F3H and extending chain spread in the whole protein;F3H contains a conserved 2OG-Fell Oxy super family domain and a conserved PLNO3176 super family domain; F3H phylogeny is in consistent with morphological species phylogeny in general.