5个与脂肪酸代谢相关基因在油菜不同生育期表达量

摘要

为了明晰与脂肪酸代谢相关基因在不同油酸含量油菜中的表达情况，以出苗后7d、5～6叶期和越冬期的叶片、花瓣和授粉后20～35 d 的种子为材料，对5个转录组分析得到的差异基因（ Bna0691280， Bna0421530， Bna0547440，Bna0799930和Bna0764010）在高油酸油菜近等基因系中的表达情况进行研究。结果发现，在营养生长期（出苗后7 d、5～6叶期和越冬期）的叶片中，5个基因在高油酸油菜材料中的表达量均高于低油酸油菜中的表达量。在花中除Bna0547440和Bna0764010外，其它3个基因在高油酸油菜中的表达量高于低油酸油菜中的表达量。在授粉后20～35 d的种子中除Bna0799930外，其余4个基因在高油酸油菜材料中的表达量均低于低油酸油菜中的表达量。研究结果可为高油酸油菜育种和分子机理研究提供参考。%In order to understand the expression of fatty acid metabolism-related genes in rapeseed materials with different oleic acid contents, in this study, the leaves of 7-day seedlings, in 5-6 leaf stage and overwintering period , petals and the seeds of 20-35 days after pollination were used as materials , and the expression of 5 differential genes ( Bna0691280, Bna0421530, Bna0547440, Bna0799930 and Bna0764010) which obtained by transcriptome analysis of high oleic rapeseed near-isogenic lines were studied .The results showed that:in the leaves , the expression levels in high oleic rapeseed material were higher than those in the low oleic rape -seed.As for the expression in flowers , except for Bna0547440 and Bna0764010, the expression levels of the other 3 genes in high ole-ic rapeseed material were higher than those in low oleic rapeseed .While those in the seeds of 20-35 days after pollination , the ex-pression levels in the high-oleic rapeseed were lower than the expression in the low-oleic rapeseed except Bna0799930.The results of this study can be used for the breeding study of molecular mechanism of high oleic rapeseed .