In order to obtain the optimum condition of the extraction and shrimp larvae toxicities of the polysaccharide from Dendrobium moniliforme from Huohan,the effect of the extraction times,extracting time,solid-liquid ratio and extraction temperature on the yield of the polysaccharides from the stems of D.moniliforme were investigated by single factor experiments and orthogonal experiments,and shrimp larvae toxicity of the polysaccharide was evaluated by corrected mortality.The decreasing order of the four influencing factors affecting the polysaccharide yield were successively extraction temperature,extracting time,solid-liquid ratio and extraction times.The orthogonal experimental results showed that the optimal condition were extraction temperature,60℃;solid-liquid ration,1:40;extracting time,2 h and extraction times,3.Being administered for 4 h,8 h and 12 h,the LD50 values of the crude were 1.03,0.66 and 0.38 mg/mL respectively.Under this condition,the polysaccharide content was 25.24%.Remarkable shrimp larvae toxicity was showed by the polysaccharide of wilding D.moniliforme from huoshan.%基于正交试验探究提取次数、料液比、提取时间及温度对多糖提取的影响.以卤虫的校正死亡率及LD50值评价铜皮石斛多糖的卤虫毒活性.实验结果显示,对铜皮石斛多糖提取的影响因素从小到大依次为:提取次数<料液比<提取时间<温度,最佳提取条件为料液比1:40、提取2 h、浸提温度60 ℃、提取3次.铜皮石斛多糖卤虫毒活性研究表明,在4 h、8 h和12 h的LD50值分别为1.03、0.66和0.38 mg/mL,相同给药时间内,多糖对卤虫毒性的强弱随浓度的增高而增高,表现出明显的量效关系.在最优提取条件下测得霍山产铜皮石斛多糖含量为25.24%;铜皮石斛多糖具有显著的卤虫毒活性.
展开▼
