[目的]研究一种可靠并广泛适用于各种病原真菌的单孢分离技术.[方法]以玉米小斑病菌的单孢分离为例,并综合改进其他单孢分离方法,通过组织分离、孢悬液涂布、挑针转移3个步骤,挑取分散在清水琼脂平板上已经萌发的孢子获得单孢.[结果]采用该法对多种病害标本进行单孢分离,成功率都在92%以上,部分达到100%,单个菌丝片段的分离成功率达到60%以上.[结论]多种真菌的分离表明,该法操作简便,材料设备简单,结果可靠,成功率高,适用性广.%[ Objective ] The isolation technique of single spore, which was suitable for various pathogenic fungi, was researched. [ Method ] The isolation technique of single spore of Bipolaria maydis was improved and the single spore, which had germinated and scattered in the water agar plate, was picked up after three steps: tissue isolation, coating spore suspension and removal of dissecting needle. [ Result] The single spore of most of samples was isolated with the technique, which successful rate was over 92％ and partially 100％; the successful rate of the isolation of single hyphal fragment was over 60％. [ Conclusion] The experimental result showed that the method was easy to operation, the requirement to material and equipment was simple, the result was reliable, the successful rate was high and the approach of its applicability was wide.
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