利用细胞壁降解酶(CWDEs)是镰刀菌(Fusarium sp.)侵染寄主的主要手段之一,不同种类的镰刀菌在致病过程中起主要作用的降解酶种类有所不同.以山西省黄芪(Astragalus L.)根腐病的优势病原菌锐顶镰刀菌[F.acuminatum(Ellis et Everhart)Wr.]、腐皮镰刀菌[F.solani(Mart)Sacc.]和尖孢镰刀菌(F.oxysporum Schlecht)为研究对象,对其活体外诱导培养产生的主要细胞壁降解酶(多聚半乳糖醛酸酶、聚甲基半乳糖醛酸酶、多聚半乳糖醛酸反式消除酶、果胶甲基反式消除酶、内切1,4-β-D葡聚糖酶、β-葡萄糖苷酶)及其变化规律进行了比较.结果表明,3种根腐病菌均能产生多聚半乳糖醛酸酶、聚甲基半乳糖醛酸酶、多聚半乳糖醛酸反式消除酶、果胶甲基反式消除酶、内切1,4-β-D葡聚糖酶和 β-葡萄糖苷酶6种CWDEs,但不同的致病菌产生各种酶的活性大小和变化趋势具有明显差异.该结果为深入研究CWDEs在根腐病菌侵染黄芪过程中的致病作用奠定了基础.%Producing cell wall degrading enzymes (CWDEs) is one of the main means of the Fusarium sp. infecting its host plants. Different kinds of Fasarium may have different CWDEs which are more active than other CWDEs in the infection pro-cess. In this paper, research was made into the main CWDEs produced by the dominant pathogens of Astragalus L. root rot in Shanxi province such as F. acuminatum (Ellis et Everhart) Wr.,F. solani (Mart) Sacc.and F. oxysporum Schlecht and the rules of their changes. A series of CWDEs including polygalacturonase (PG),polymethylgalacturonase (PMG),polygalacturonic acid transeliminase (PGTE),pectin methyltranseliminase (PMTE),carboxymethyl cellulose (Cx),β-glucosidase (βG),were de-tected by the induction and culture in vitro. The results show that the 3 kinds of root rot pathogens all produced PG,PMG, PGTE,PMTE,Cx and βG,but significant differences existed in the activities and dynamic changes of each of the various en-zymes produced by these pathogens. The above results laid the foundation for further study on the pathogenicity of CWDEs in the infection of root rot pathogens.
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