Separation of phytosterols from deodorizer distillate by microbial fermentation was carried out for the first time and crude phytosterols was extracted successfully from the fermented broth. The influences of main components in culture medium on the cell growth of Candida tropicalis K12, consumption of fatty acid and releasing of sterols from deodorizer distillate were studied in this work. The optimal culture medium was:deodorizer distillate of soybean oil 40.0 g/L, yeast extract 1.5 g/L, K2HPO4 2.5 g/L and MgSO4 1.0 g/L. After fermentation of C. tropicalis K12 with the medium above, the maximum dry cell weight was 15.6 g/L, the maximum consumption rate of fatty acids was 42.3%and the maximum rat of sterol extraction from deodorizer distillate was 44.1 %. This study had proved that separation of phytosterols from deodorizer distillate by microbial fermentation was successful and feasible , which provided a new approach to produce of phytosterols without environmental pollution.% 首次采用微生物发酵法来分离脱臭馏出物中植物甾醇，并成功地从发酵液中提取出了粗甾醇。研究脱臭馏出物发酵过程中脱臭馏出物添加量、酵母粉、无机盐对热带假丝酵母K12菌体生长、脂肪酸消耗和甾醇释放的影响，确定适宜的发酵培养基为：大豆脱臭馏出物40.0 g/L、酵母粉1.5 g/L、K2HPO42.5 g/L、MgSO41.0 g/L。经过热带假丝酵母K12发酵，获得最高菌体干重为15.6 g/L，最大脂肪酸消耗率为42.3%，最高甾醇释放率为44.1%。本研究证实了微生物发酵法分离提取脱臭馏出物中植物甾醇的可行性，为植物甾醇的绿色清洁生产提供了新的途径。
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