利用响应面分析法优化葛渣异黄酮提取工艺

摘要

The conditions of ultrasonic assisted extraction of isoflavones from pueraria root residue were optimized based on response surface mothodology (RSM).On the basis of single-factor test, the mathematical regression model was established on the dependent variable (extraction rate of isoflavones ) and independent variables (material-to-liquid ratio,extraction temperature and extraction time) by Box-Benhnken design and response surface methodology with three factors at three levels. The optimum extraction conditions were as follows:ethanol concentration 70%,material-to-liquid ratio 1︰17 (g︰mL), extraction temperature 70℃, extraction time 26 min, extracting for 2 times. Under these conditions, the extraction rate of isoflavones from pueraria root residue is up to 96.3%, which has no significant difference with the prediction value (P>0.05).%　　利用响应面分析法（RSM）对生产葛粉后的葛渣中异黄酮的超声波辅助提取条件进行优化。在单因素试验基础上，选取料液比、提取温度和超声提取时间三因素为自变量，以葛渣异黄酮提取率为响应值，根据Box-Benhnken试验设计原理采用三因素三水平的响应面分析法建立回归模型，得到超声波辅助提取葛渣异黄酮的最佳条件为乙醇浓度70%，料液比1︰17（g︰mL），提取温度70℃，提取时间26 min，提取两次，异黄酮提取率可达96.3%，与模型预测理论值差异不显著（P＞0.05）。