Casein was the main protein in milk, not only provided the necessary amino acids for the organism growth, but also can get a series of bioactive peptides had important physiological functions after enzymolysis , and anticoagulant peptide had little side-effect and observably anticoagulant effect was one of them , it can be used as a potential drug to cure cardiovascular and cerebrovascular diseases. In this experiment , In order to obtain high activity anticoagulant peptide from yak casein protein, an method that enzyme hydrolysis casein protein was used and established the optimal preparation conditions through sequential steps of screening single-factor and designing orthogonal test. It was determined as enzymolysis temperature 30℃, pH6.2, substrate concentration 4%,the amount of enzyme 1.75%, reaction time 3 h. The anticoagulant potency unit fate could reach 837.4 U/mg under the optimized conditions.%酪蛋白为牛乳中主要的蛋白形式，不仅能为生物体生长发育提供必需的氨基酸，而且酶解后可得到一系列具有重要生理功能的生物活性肽，抗凝血肽就是其中一种，毒副作用小、抗凝血效果明显，可作为心脑血管疾病治疗的潜在药物。本试验以牦牛乳酪蛋白为原料，采用胰蛋白酶酶解酪蛋白制备抗凝血肽，通过筛选显著单因素和设计正交试验，确定了牦牛乳酪蛋白抗凝血肽最佳制备条件为：温度为30℃、pH为6.2、底物浓度为4%、酶底比为1.75%、反应时间为3 h。在该条件下所制备的抗凝血肽的抗凝血效价单位数可达837.4 U/mg。
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