LC-MS/MS测定黄鱼中碱性嫩黄O及酸性橙Ⅱ

摘要

建立高效液相色谱-串联四极杆质谱正负离子切换模式同时快速测定黄鱼中黄色工业染料碱性嫩黄O及酸性橙Ⅱ的检测方法。样品匀质后，经乙腈提取，盐析，-20℃冷冻脱脂，低温离心后，取乙腈层采用高效液相色谱-串联质谱法分离、测定。结果表明碱性嫩黄O在0.5滋g/kg~10滋g/kg、酸性橙Ⅱ在5滋g/kg~100滋g/kg范围内呈良好的线性关系，高中低浓度回收率在70%~110%，相对标准偏差（n=5）小于15%，碱性嫩黄O、酸性橙Ⅱ检出限分别为0.5、5滋g/kg。本方法适用于黄鱼中违禁工业染料碱性嫩黄O及酸性橙Ⅱ的测定。%A high performance liquid chromatography tandem quadrupole mass spectrometry method was developed for rapid determination of Auramine O and Acid orangeⅡin yellow croaker. The sample was extracted by acetonitrile, the fat was removed through freezing at-20℃, then analyzed by LC-MS/MS. The results showed good linearity in the ranges of 0.5 μg/kg-10μg/kg,5μg/kg-100μg/kg for Auramine O and Acid orangeⅡ, respectively. The recoveries were between 70%and 110%, and the value of the RSD (n=5) was lower than 15%for the both. The limit of quantitative (LOQ) for Auramine O and Acid orangeⅡwas 0.5μg/kg and 5μg/kg, separately. The method was suitable to detect Auramine O and Acid orangeⅡas two banned industrial dyes in yellow croaker.