微生物法测定食品中的烟酰胺

摘要

The Lactobacillus plantarum (ATCC 8014)has great sensitivity for nicotinamide in materials. According to this specificity, it can be assay nicotinamide in samples. Prepare standard concentrate-response curve by plotting absorbance reading for each level of standard solution. Determine the amount of nicotinamide activity for each level of assay solution by interpolating from standard curve. The precision were 1.0 %, 0.7 %and 1.0 %. The RSD was 0.368 2 %. The recoveries were all above 97 %.%利用植物乳杆菌ATCC 8014对烟酰胺的特异性，定量测出样品中烟酰胺的含量。以不同浓度标准溶液的吸光度相对于各浓度水平标准物质的浓度绘制标准曲线，再根据标准曲线计算出试样烟酰胺含量。方法精密度分别为1.0%、0.7%及1.0%，相对标准偏差为0.3682%，回收率在97%以上。