乳酸菌发酵法检测鲜乳和乳粉中的两种抗生素残留

摘要

Lactic acid bacteria fermentation can be restricted by antibiotics. The method of lactic acid bacteria fermentation for determination of two antibiotic (penicillin and ceftiofur) residues in milk and milk powder was established. The detection limits of two antibiotics were obtained by adding antibiotics to non-anti-milk , inoculating lactic acid bacteria and determining pH during fermentation. The results showed that , when milk was used as background, detection limits of penicillin and ceftiofur were 0.08 U/mL and 0.08 μg/mL, when milk powder was used as background, detection limits of penicillin and ceftiofur were 0.1 U/mL and 0.08μg/mL. This method is simple, rapid, and inexpensive, and suitable for promotion.%根据抗生素能抑制乳酸菌发酵的原理，建立了用乳酸菌发酵检测鲜乳及乳粉中青霉素和头孢噻呋残留的方法。通过向无抗乳中添加抗生素，接种乳酸菌，测定发酵过程中的pH，得出两种抗生素的检测限。结果表明：以鲜乳为本底时，青霉素和头孢噻呋的检测限分别为0.08 U/mL、0.08μg/mL；以乳粉为本底时，青霉素和头孢噻呋的检测限分别为0.1 U/mL、0.08μg/mL。该方法简单、快速、价格低廉，适于推广。