乌鸡肽免疫调节作用的研究

摘要

The basic physical and chemical indicators of Black-bone chicken peptide were measured,and its immunomodulatory activity was study. The effect of Black-bone chicken peptide on the proliferation of murine splenocytes was detected by WST-8. The effects of Black-bone chicken peptide on phagocytic activity and the No production by macrophages were studied in order to evaluate the cell viability of mouse peritoneal. The relative molecular weight of Black-bone chicken peptide was less than 1 000 U. Black-bone chicken peptide significantly stimulated ConA- and LPS-stimulated splenocyte proliferation,and significantly promoted murine peritoneal macrophage phagocytic ability of neutral red and the NO production at 100μg/mL. Black-bone chicken peptide has good immunomodulatory effect.%对乌鸡肽的基本理化指标进行了测定，并对其免疫调节活性进行研究。应用WST-8法检测不同浓度乌鸡肽对小鼠脾淋巴细胞增殖的影响，以吞噬能力和NO的生成量为指标评价乌鸡肽对小鼠腹腔巨噬细胞活力的影响。乌鸡肽主要含有相对分子量低于1000 U的短肽，对ConA诱导的T-淋巴细胞增殖和LPS诱导的B-淋巴细胞增殖均有显著的促进作用。浓度为100μg/mL时，显著促进小鼠腹腔巨噬细胞吞噬能中性红的能力和NO生成。乌鸡肽具有较好的增强免疫调节的能力。