温和压力对面包酵母CICC1447谷胱甘肽合成的影响

摘要

研究了保压时间、压力和升降压速率对面包酵母CICC1447谷胱甘肽（GSH）生物合成能力的影响。以高纯空气（O2∶N2=21∶79）为加压介质，通过单因素实验确定了最优加压条件。实验结果表明，在温和压力刺激条件下酵母菌受可产生应激性反应。谷胱甘肽在最适条件（保压时间6 h、压力0.5 MPa、升降压速率0.05 MPa/min～0.10 MPa/min）下的最大产量比常压下提高了17.21%。结果表明温和压力可以提高微生物重要应激产物谷胱甘肽的产量。%The effects of pressure, treatment time and pressurization and depressurization rates on the glutathione biosynthesis of Saccharomyces cerevisiae CICC1447 were researched. The optimum conditions were established by single factor test using high purity air (O2∶N2=21∶79) as the pressurizing medium. The results indicate that the stress reaction occurred after being stimulated for the Yeast cells under mild pressure. The maximum yield of glutathione (GSH) under optimized conditions (processing time 6h, pressure value 0.5 MPa, and pressurization and depressurization rates 0.05 MPa/min~0.10 MPa/min) was increased by 17.21 % than normal pressure. The results show that mild pressure can improve the yield of GSH, which is one of the important stress metabolic productions of plenty of microorganism.