黄海海燕体壁胶原蛋白肽的制备及其抗氧化活性

摘要

Collagen was extracted from the body wall of starfish Asterina pectinifera, and the purity was detected using SDS-PAGE electrophoresis. Then the collagen was hydrolyzed with pepsin and papain under optimum conditions. After ultrafiltrating with the 10 ku membrane , the collagen peptides molecular weight less than 10 ku was prepared. And the antioxidant activity of collagen peptides was detected using electron spin resonance (ESR) spectroscopy. The results indicated that the extracted collagen of high purity, with a complete triple helix;the hydrate degree of collagen treated with pepsin and papain were (5.46±0.14)%and (5.04± 0.37)%, respectively;DPPH radical scavenging ability increased with the increasing concentration of collagen peptides, at the concentration of 20 mg/mL, collagen peptides (pepsin group) and collagen peptides (papain group) on DPPH scavenging rate were (52.10±0.10)%and (46.78±0.09)%, respectively.%以黄海海燕体壁为原料提取胶原蛋白，采用SDS-PAGE电泳检测胶原蛋白的纯度；再分别用胃蛋白酶和木瓜蛋白酶在各自最适条件下水解胶原蛋白，经10 ku的超滤膜超滤后，制备出分子量小于10 ku的胶原蛋白肽；并应用电子自旋共振波谱技术检测胶原蛋白肽的抗氧化活性。结果表明，提取的胶原蛋白纯度高，具有完整三螺旋结构；胃蛋白酶与木瓜蛋白酶对胶原蛋白的水解度分别为（5.46±0.14）%和（5.04±0.37）%；胶原蛋白肽对DPPH自由基的清除能力随着浓度的升高而提高，浓度为20 mg/mL，胶原蛋白肽（胃蛋白酶组）和胶原蛋白肽（木瓜蛋白酶组）对DPPH的清除率分别为（52.10±0.10）%和（46.78±0.09）%。