浒苔多糖脱蛋白工艺的考察

摘要

The method was investigated to remove protein from polysaccharide of Enteromorpha prolifera.-The effect of deproteinization with different methods such as TCA, Sevag, enzyme, and enzyme-Sevag method was evaluated in terms of the ratio of protein removal and polysaccharide loss. The result showed that enzyme-Sevag method was better than others with the higher protein removal. The optimum technological conditions of deproteinization were:enzyme usage 3%, time 3 h, temperature at 45℃, pH5.0, and Sevag deproteinization 2 times. After treatment with optimum conditions , the ratio of deproteinization and polysaccharide loss were 89.73 % and 27.46 %, respectively. Enzyme-Sevag method is effective and alternative and can be used for removing protein from polysaccharide of Enteromorpha prolifera.%研究浒苔多糖的脱蛋白方法与工艺。以蛋白脱除率和多糖损失率作为为指标比较三氯乙酸（TCA）法、Sevag法、酶法及酶-Sevag法脱蛋白的效果。结果表明：酶-Sevag法脱蛋白效果较好，其最佳工艺条件为：酶用量3%，酶解时间3 h，酶解温度45℃，pH 5.0，Sevag法脱蛋白次数2次，在此条件下，蛋白质脱除率和多糖损失率分别为89.73%和27.46%。酶-Sevag法是去除浒苔粗多糖中蛋白质较理想的方法，该优化工艺可用于浒苔粗多糖的脱蛋白。