灵芝中灵芝酸的测定研究

摘要

The HPLC determination method for the determination of Ganoderma lucidum acid was established. The result showed that the maximum standard deviation of cholesterol content was 1.36%under this method for determination and Cholesterol content displayed an excellent linear relationship , the standard curve equation was Y=625.71x+82.857(R=0.998 2). The spike recoveries for cholesterol were between 97.04%and 102.64%, the method was feasibility and accurate for using to determine Ganoderma lucidum acid, the average contents of Ganoderma lucidum acid was 1.03 mg/g by HPLC determination.%利用HPLC法测定灵芝中灵芝酸含量的方法，此方法测定灵芝中灵芝酸含量重复性试验最大标准偏差为1.36%，峰面积与灵芝酸标品线性关系良好，标准曲线方程为Y=625.71x+82.857（R=0.9982），加标回收率在97.04%~102.64%之间，此方法用于灵芝中灵芝酸含量准确、可行；经测定灵芝中灵芝酸为含量1.03 mg/g。