不同方法提取的草鱼鱼皮胶原蛋白性质比较

摘要

Extract collagen of grass carp's skin with acid, alkali ,enzyme and water method, the result of collagen's extraction yield in different method was as follow:acid>alkali>enzyme>water. The result of collagen's in different method was as follow: alkali >acid >water >enzyme. The result of collagen's SDS -PAGE electrophoresis was: the ribbons from water and alkali appeared in β-dipolymer and γ-tripolymer ribbons position, the ribbons from enzyme and acid no appearance, butα-chain's ribbons from enzyme and acid marks significantly thicker than ribbons from water and alkali. The result of collagen's UV scanning spectrum was:UV absorption intensity from acid and alkali were greater than that from water and enzyme , the maximum UV absorption left side from alkali and enzyme appeared some miscellaneous peaks. The result of collagen's FI-IR spectrum was: according with the characteristic absorption peak. The collagen extracted in four methods all have characteristic absorption peaks which are amideⅠ, amideⅡ, amideⅢ, and their position are similar to each other, It was in accordance withⅠcollagen's characteristic absorption peak.%采用酸法、碱法、酶法、水法从鱼皮中提取胶原蛋白，对其性质进行分析比较。结果表明，提取率，酸法＞酶法＞碱法＞水法；热变性温度，水法＞酶法＞碱法＞酸法；水法和碱法提取的胶原蛋白SDS-PAGE电泳图谱条带中出现β二聚体和γ三聚体，酶法和酸法没有出现，但是酶法和酸法的α链位置明显色带浓于水法和碱法；紫外吸收光谱中，酸法和碱法的吸收峰强度大于水法和酶法，碱法和酶法左侧出现了杂峰；红外光谱显示，碱法提取的鱼皮胶原蛋白不具备酰胺带特征吸收峰，其二级结构已破坏，酸法、酶法和水法提取的胶原蛋白均存在酰胺带特征吸收峰，而且出现位置相似，符合胶原蛋白特征吸收峰。