大海马中总甾醇提取工艺优化及成分分离

摘要

In the present work, we optimized the extracting conditions of total sterol in Hippocampus kuda by Box-Behnken response surface method according to the difference in absorbance of vanillin perchloric acid color reaction. Three factors associated with extraction effect were determined , including extraction temperature, extraction time and ratio of material to liquid. The content of total sterol in the hippocampus was also determined with a standard of cholesterol. The results showed that the highest total sterol extraction amount was obtained to 5.17 mg/g when the extraction temperature was at 79℃, extraction time at 7.7 h, and the ratio of solid to liquid at 80 mL/g. The purified sample was further identified by high performance liquid chromatography (HPLC). This paper provided a preliminary basis for the extraction and separation of the sterol composition in Hippocampus kuda.%根据香草醛-高氯酸显色反应后样品吸光度的差异，用Box-Behnken响应面法优化了海马中甾醇的提取条件。主要确定了提取温度、提取时间及料液比3个因素对甾醇提取效果的影响。并以胆固醇为标准品，测定了海马中总甾醇的含量。结果表明：提取温度为79℃，提取时间为7.7 h，料液比为80 ml/g时，海马中的总甾醇提取量最高，提取量为5.17 mg/g。通过高效液相分析，初步分离海马总甾醇提取物，为海马中甾醇成分的提取和分离提供了初步的依据。