利用JMP软件优化威兰胶发酵培养基

摘要

The statistical analysis software JMP(its function includes Plackett-Burman design, Factorial design, Central compositon design ,Prediction Profiler et al.)was applied to optimize the fermentation medium of welan gum by Alcaligenes sp.Y5. The optimal composition of the medium was determined as glucose 52.9 g/L, beef extract 4.0 g/L,K2HPO4·7H2O 2.2g/L,MgSO4·7H2O 0.1 g/L,CaCO3 2.0g/L,Tween80 0.5 g/L by using the optimised medium, the welan gum production increased from 15.72 g/L to 26.58 g/L,with an increase of 69.08%.%利用统计分析软件JMP的试验设计（DOE）功能（包括Plackett-Burman设计、析因设计、中心组合设计，预测刻画等）对威兰胶发酵菌株Alcaligenes sp.Y5的发酵培养基进行了优化，得到一组最佳发酵培养基配方：葡萄糖52.9 g/L、牛肉膏4.0 g/L、K2HPO4·7H2O 2.2g/L、MgSO4·7H2O 0.1g/L，CaCO32.0 g/L，吐温800.5 g/L。优化后威兰胶产量由初始的15.72 g/L提高到26.58 g/L，提高了69.08%。