山核桃外蒲壳中胡桃醌的提取工艺优化研究

摘要

采用单因素试验设计、正交试验设计法对山核桃外蒲壳中胡桃醌提取进行了优化，获得了料液比、提取温度、提取时间等参数，并建立了参数方程。由试验结果可知，采用超声波提取法、直接浸提粉末样品，甲醇提取率最高，考虑到安全问题选择乙醇为提取溶剂，提取溶剂乙醇效果好于甲醇和石油醚，且95%乙醇效果好于50%和70%乙醇，料液比以1∶40（g/mL）、提取时间和温度分别为50 min和40℃提取效果较佳。正交试验结果表明最佳工艺为B2A3C3，即料液比1∶40（g/mL），提取时间50 min，提取温度50℃时，提取量为0.0625 mg/mL，是非正交试验结果平均值的1.3倍。%The single factor and orthogonal experiment design methods were utilized to optimize the extraction con ditions of juglone from Hickory Hull. The results showed that the ultrasonic extraction method, methanol as ex-traction reagent had higher extraction rate, but for safety consideration, ethanol was better than methanol and petroleum ether, and 95%ethanol is better than 50%and 70%ethanol, the material liquid ratio of 1∶40(g/mL), extraction time and temperature of 50 min and 40℃showed higher extraction rate. Orthogonal experiment re-sults showed that the optimal conditions of B2A3C3, namely the material liquid ratio of 1∶40 (g/mL), extraction time and temperature of 50 min and 50℃had the optimal extraction rate of 0.062 5 mg/mL, which was 1.3 times than the average value of single factor experiments.