荧光免疫层析检测牛奶中的生物素含量

摘要

A composition with a character and uniform size which contained fluorescent microspheres as a novel lable was obtained by an EDC/NHS two-step method.The biotin in milk was detected by the immunofluores-cence chromatography which had this compositoin as a probe.The method showed good specificity with the de-tection limit of 3 ng/mL,the linear range of 5 ng/mL-100 ng/mL,the recovery of 90%-110%and the precision CV less than10 %;the correlation coefficient of measuring value was 0.975 compared with microbiological method.%以荧光微球作为新型标记物，采用EDC/NHS两步法偶联，制备荧光微球链霉亲合素复合物。复合物颗粒均一、性质稳定。以其作为探针，建立检测牛奶中生物素含量的荧光免疫层析法。本方法特异性良好，最低检出限低至3 ng/mL，线性范围5 ng/mL～100 ng/mL，回收率90%～110%，精密度CV＜10%；与微生物法对比，测值相关系数为0.975。