荸荠提取物对乙醇脱氢酶活性的影响

摘要

To study the impact of the extract from Chinese water chestnuts on the alcohol dehydrogenase(ADH) activity. With the active target of ADH, the improved Valle&Hoch method was used to detect the influence of various extract from water chestnuts on ADH activity in vitro. Male mice were randomly divided into five groups, which was used to study the influence of extract from water chestnuts on alcohol concentration and ADH activity in blood and liver of mice after oral administration of alcohol. And the alcohol concentrations in mice were deter-mined by the method of GC-FID. Compared with normal saline, water extract of Chinese water chestnuts have significantly increased the ADH activity. And the value of ADH activity was 45.2 % at the concentration of 0.5 g/mL. However, the ethanol extracts of water chestnuts could obviously inhibit the ADH activity. After oral administration of aqueous extract from Chinese Water Chestnut, the ADH active in mice blood rose to the peak value in the period of 0.5 h-1.5 h in the model group. At the same time, the ADH activity in mice liver rose to peak value during 0.5 h-2.5 h. Although the changing trend of ADH activity in model group was similar with the trend in extract group, the ADH activity in model group were lower than in extract group. Besides, the alcohol concentration in blood rose to the maximum value during 0.5 h-12 h in the model group, which was similar about the changing trend in the two group. By the usage of pharmacokinetics formula, the elimination half-life was 7.41, 5.63, 3.02 and 2.56 h in the model group and different extract groups, respectively. The results showed that the aqueous extract of Chinese water chestnut could reduce the alcohol concentration by activating ADH in vitro. Fur-thermore, the persistent activation of ADH was exhibited by the aqueous extract.%探讨荸荠提取物对乙醇脱氢酶（ADH）活性的影响及其对小鼠急性酒精中毒的改善作用。以乙醇脱氢酶为作用靶点，采用瓦勒-霍赫法（Valle&Hoch）检测不同荸荠提取物对乙醇脱氢酶活性的影响。将雄性昆明小鼠随机分成5组，检测小鼠酒后不同时间点血液和肝脏中乙醇脱氢酶活性变化。同时利用气相色谱（GC-FID）测定不同时间点小鼠血清中乙醇浓度。结果表明，荸荠水提物对乙醇脱氢酶活性有显著激活作用，当浓度为0.5 g/mL时，对乙醇脱氢酶的激活率为45.2%。而荸荠醇提物对乙醇脱氢酶有显著抑制作用。通过口灌给予不同浓度荸荠水提取物后，小鼠血清中ADH活性在灌胃后0.5 h~1.5 h内达到高峰，小鼠酒后肝脏中ADH活性在0.5 h~2.5 h内达到峰值，与模型组趋势一致。模型组和荸荠水提物灌胃组小鼠血液中乙醇浓度均在乙醇灌胃后0.5 h达到峰值，但模型组小鼠血液中乙醇峰浓度较荸荠提取物灌胃组高。在0.5 h~12 h内，模型组和水提取物组小鼠血液中乙醇浓度均呈下降趋势。乙醇在模型组和水提物灌胃组小鼠体内消除半衰期分别为7.41、5.63、3.02、2.56 h，表明水提物灌胃能明显增强小鼠体内乙醇的消除能力。结论：荸荠水提取物对ADH具有显著激活作用，同时显著增强小鼠血液和肝脏中ADH活性。