A stimulate method for determination of aflatoxin and aflatoxin-like compounds in Aspergilli metabolite was developed by high performance liquid chromatography-linear ion trap mass spectrometry. A. parasiticus (strain 3.124) which produce AFB1 was cultivated by PDA solid medium, then the metabolites of strain 3.124 was analyzed by mass analyzer of QTrap(positive ion mode, multiple reaction monitoring(MRM)) after extraction and purification, and found five mycotoxins of AFB2、AFG1、AFG2、MST and ST through the MRM methods predicted by MS2 of AFB1 and structural formula of aflatoxin-like compounds, and was confirmed by standard. The linear range of AFB2 and AFG2 was 0.5μg/L-40μg/L, the other 4 metabolites showed good linearity in the range of 0.2μg/L-40μg/L with the correlation coefficients more than 0.99. The recoveries of in-ter-day ranged from 81.3%to 92.3%with the relative standard deviations of 3.4%-6.2%, The recoveries of intra-day ranged from 82.1%to 91.7%with the relative standard deviations of 3.8%-7.7%. A new method for rapid determination of aflatoxin and aflatoxin-like compounds in Aspergilli metabolite was provided.%建立了同时检测曲霉菌代谢物中黄曲霉毒素和同系物的高效液相色谱-线性离子阱质谱测定方法。产黄曲霉毒素B1（AFB1）寄生曲霉（菌株3.124）经PDA固体培养基培养，提取净化后经线性离子阱（QTrap）质量分析器分析（正离子模式，多反应检测），以AFB1二级碎片（MS2）信息和其同系物分子式信息预测代谢物MRM方法，检出3.124代谢物中黄曲霉毒素B2（AFB2）、黄曲霉毒素G1（AFG1）、黄曲霉毒素G2（AFG2）、O-甲基杂色曲霉素（MST）、杂色曲霉素（ST）5种真菌毒素，并用标准品进行了确证。结果表明，AFB2和AFG2在0.5μg/L～40μg/L，其它4种代谢物在0.2μg/L～40μg/L范围内线型关系良好，相关系数均大于0.99。本方法6种代谢物日内回收率81.3%～92.3%，相对标准偏差（RSD）为3.4%～6.2%；日间回收率82.1%～91.7%， RSD为3.8%～7.7%。该方法为快速检测霉菌代谢物中的同系物提供新的方法。
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