对八角内生真菌Chaetomium globosum BJEF13产抗氧化活性物质的培养条件进行了研究。在筛选确定基础培养基的基础上，采用单因素试验方法考察了培养基组成及摇瓶发酵条件对菌株发酵液抗氧化活性、菌株生长的影响。基础培养基的筛选结果表明，马铃薯葡萄糖培养基、察氏培养基、马丁培养基、豆芽汁蔗糖培养基等4种常用真菌培养基中，察氏培养基优于其他3种。培养基组成和发酵条件的研究结果表明，当以4%葡萄糖、0.4%的蛋白胨、0.1%K2HPO4、0.05%KCl、0.05%MgSO4和0.001%FeSO4作为摇瓶发酵培养基时，接种量为10%，装液量为100 mL/250 mL，在初始pH为7.5、28℃、150 r/min条件下振荡培养7 d，该菌株的生长和培养液的抗氧化活性可以达到较好的水平，其生物量平均干重达0.6826 g/100 mL，5倍稀释的乙酸乙酯萃取液对DPPH自由基的清除率可达57.51%。%Influences of cultural conditions on the production of antioxidant materials by the endophytic fungal Chaetomium globosum BJEF13 isolated from Illicium verum were studied. After screening the basic culture medium, influences of compositions of the cultural medium and fermentation conditions to the dry weight of mycelia and DPPH· scavenging activity were investigated using single factor experiment. The basic culture medium screening results indicated that Czapek culture medium was more suitable for the strain than the other three media, the PD culture medium, Martin culture medium and BSS culture medium. The results of single factor experiments for components of Czapek culture medium indicated that the suitable cultural conditions for the mycelia growth and production of antioxidant materials were as followed:4%glucose, 0.4%peptone, 0.1%K2HPO4, 0.05%KCl, 0.05%MgSO4, 0.001%FeSO4, initial pH 7.5, temperature 28℃, inoculation amount 10%, liquid volume 100 mL/250 mL, shaking speed 150 r/min and cultural time 7 days. Under the suitable cultural conditions, the dry weight of mycelia and DPPH· scavenging activity of 5-fold dilution of acetate extract of the broth were 0.682 6 g/100 mL and 57.51%, respectively.
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