采用高效液相色谱法(HPLC)对小米中非法染色姜黄素进行定性定量测定,并对其不确定度进行分析.以乙腈-4%冰乙酸(45+55)为流动相,流速1.0 mL/min,经C18柱分析,检测波长425 nm.采用外标法定量,姜黄素在0.585μg/mL~4.88μg/mL范围内线性关系良好,相关系数r2=0.9999,平均回收率92%,检出限为0.08 mg/kg,整个试验的合成不确定度为1.937%,扩展不确定度为X=(7.15±0.29)mg/kg;k=2.本方法操作简单,重复性好,检出限低,可用于小米中姜黄素非法染色的测定.%High performance liquid chromatography(HPLC) was used to qualitatively and quantitatively deter-mine curcumin in millet and its uncertainty was analyzed. Acetonitrile-4%acetic acid (45+55) solution was used as the mobile phase, and 1.0 mL/min as the flow rate, and C18 as the analysis column. Good linear rela-tionship was obtained in the concentration range of 0.585μg/mL-4.88μg/mL of curumin, with correlation coef-ficient of 0.9999. Under the optimized conditions, the average recoveries were 92%. The limit of detection(S/N=3) of curcumin was 0.08 mg/kg. The combined uncertaincy was 1.937%and the expanded uncertainty was X=(7.15±0.29)mg/kg;k=2. The method was sample, reproducible, low detection limit, and can be used to de-termine the illegal staining of curcumin in millet.
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